Facile green preparation of PLGA nanoparticles using wedelolactone: Its cytotoxicity and antimicrobial activities.

[Display omitted] • Wedelolactone, a plant compound, was loaded-poly (lactic-co-glycolic acid) into the synthesized NPs against breast cancer and bacteria. • WDL PLGA-NPs characterization by FTIR, XRD, DLS and TEM. • A better antimicrobial effect has been achieved by WDL PLGA-NPs. • WDL PLGA-NPs were significantly cytotoxicity in breast cancer cells. We synthesized a novel wedelolactone-loaded poly-lactic- co -glycolic acid nanoparticle (WDL-PLGA NPs) and evaluated its antibacterial and induce cytotoxicity against breast cancer MDA-MB-231 cell line growth. The physicochemical properties of the WDL-PLGA NPs were defined by Fourier transform infrared spectroscopy (FTIR), and dynamic scattering scanning (DLS) analyzer, and transmission microscopy (TEM) were analysis. FTIR results showed that functional groups of WDL were involved in stabilizing as capping agents. The WDL-PLGA-NPs were spherical shaped with a crystallinity of 53.1 nm in average sizes and a surface charge of −33.0 mV predicted from TEM and zeta potential, respectively. WDL-PLGA NPs exhibit significant antibacterial activity against Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia , and Pseudomonas aeruginosa. The half-inhibitory concentration (IC 50) of the formulated WDL-PLGA NPs was 23 μg/ml but 57 μg/ml in WDL in the breast cancer MDA-MB-231 cell line. The apoptotic effect, nuclear condensation, ROS generation, the loss of mitochondrial membrane potential (Δψm) of WDL-PLGA NPs were analyzed. Thus, WDL-PLGA NPs reduced the levels of Bcl-2 but increased the levels of Bax, which results in driving the cells to undergo apoptosis. Thus, the WDL-PLGA NPs were biocompatible with antibacterial and anticancer properties. [ABSTRACT FROM AUTHOR]