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Concurrent Evaluation of the Expression and Methylation of Secreted Frizzled-Related Protein 2 along with Beta-Catenin Expression in Patients with non-M3 Acute Myeloid Leukemia.
- Source :
-
Iranian Journal of Medical Sciences . May2021, Vol. 46 Issue 3, p180-188. 9p. - Publication Year :
- 2021
-
Abstract
- Background: Wnt signaling is a critical pathway for the development of acute myeloid leukemia (AML). Some studies have evaluated the expression or methylation of secreted frizzled-related protein 2 (SFRP2) as an antagonist and beta-catenin (β-catenin) as a critical mediator of this pathway. Since we found no comprehensive study on these genes in Iran, we aimed to investigate the status of both SFRP2 expression and methylation, and also β-catenin expression, in conjunction with clinical characteristics, in Iranian patients with de novo non-M3 AML. Methods: The methylation and expression of SFRP2 were determined in 188 patients with primary non-M3 AML and 60 healthy controls, who were referred to Shariati Hospital, Tehran, Iran, between January 2017 and February 2019. The methylation-specific polymerase chain reaction (PCR) and realtime quantitative PCR were used, respectively. The expression of β-catenin was explored via real-time quantitative PCR. Statistical analysis was performed using the Mann-Whitney U test (SPSS software, version 23). A P value of less than 0.05 (2-tailed) was considered significant. Results: SFRP2 mRNA showed a significant decline in the AML group compared with the controls (P<0.001). The hypermethylation of the SFRP2 promoter occurred in 25.5% (48/188) of the cases. SFRP2 expression exhibited a negative correlation with the white blood cell count (P=0.003). The expression of β-catenin increased significantly in the patients in comparison with the controls (P<0001), and a significant difference was observed between the patients, who achieved complete remission and those, who did not (P=0.046). Conclusion: The findings of this study showed that alterations in SFRP2 and β-catenin expression can be used as a potential biomarker for differentiating patients with new non-M3 AML from the controls. Additionally, an evaluation of β-catenin expression may be valuable in predicting complete remission in patients with non-M3 AML. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SECRETED frizzled-related proteins
*BIOMARKERS
*REVERSE transcriptase polymerase chain reaction
*RESEARCH
*ACADEMIC medical centers
*CYTOSKELETAL proteins
*MANN Whitney U Test
*GENE expression
*CELLULAR signal transduction
*METHYLATION
*DESCRIPTIVE statistics
*MESSENGER RNA
*POLYMERASE chain reaction
*DATA analysis software
*STATISTICAL correlation
*DISEASE remission
Subjects
Details
- Language :
- English
- ISSN :
- 02530716
- Volume :
- 46
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Iranian Journal of Medical Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 150578277
- Full Text :
- https://doi.org/10.30476/ijms.2020.84316.1396