Antioxidant activity, xanthine oxidase inhibitory activity, and compounds determination of Cipadessa baccifera leaf extract.

Cipadessa baccifera (C. baccifera) is a plant belonging to the Meliaceae family growing in tropical areas and containing terpenoids and flavonoids. Flavonoids have antioxidant properties so they can inhibit oxidoreductase enzyme activities, such as xanthine oxidase playing a significant role in uric acid biosynthesis. This study aimed to determine the antioxidant activity, xanthine oxidase inhibition activity, and the bioactive compounds in methanol extract and the most active fraction of C. baccifera leaves using LC-MS/MS. The research was carried out in the following steps: 1) extraction of C. baccifera leaves by maceration method using methanol solvent then phytochemical test, 2) fractions separation using solvents of n-hexane, ethyl acetate, butanol and water, 3) antioxidant and xanthine oxidase inhibition test of initial methanol extract and each fraction, 4) Identification of compounds using LC-MS/MS. The results showed that the methanol extract of C. baccifera contained alkaloids, flavonoids, tannins, saponins, and steroids. The ethyl acetate fraction had the highest levels of flavonoids, antioxidant activity, and xanthine oxidase inhibitory activity, and the LC-MS/MS analysis indicated that this fraction contained quercimeritrin, kaempferol 3-0-β-D-glucopyranoside, oxofangchirine, and glehlinoside B. [ABSTRACT FROM AUTHOR]