多巴胺联合NE对感染性休克致急性肝损伤大鼠肝功能、炎性因子及NF-κB p65蛋白的影响.

To investigate the effects of DA combined with NE on liver function, inflammatory factors and NF-NF-kB p65 protein in rats with acute liver injury caused by septic shock, in order to provide a certain experimental basis for clinical treatment. A total of 48 SPF-grade healthy male SD rats were chosen as research subjects and were randomly divided into four groups,i.e. the control group (n=12), LPS group (n=12), NE group (n=12), and DA+NE group (n=12). The septic shock models were established in the LPS, NE and DA+NE group. The NE group received intravenous infusion of norepinephrine, and the DA+NE group received intravenous infusion of DA on the basis of the NE group's therapy. The liver function, inflammatory factors and NF-kB p65 protein levels of rats in each group were detected. Compared with the control group, the serum AST and ALT levels of the LPS, NE and DA+NE group were significantly increased (P＜0.05). Compared with the LPS group, the serum AST and ALT levels of the rats in the NE and DA+NE groups were decreased to varying degrees(P<0.05). Compared with the NE group, the serum AST and ALT levels of the DA+NE group decreased more significantly(P<0.05). Compared with the control group, the serum IL-6 and TNF-k levels in the LPS, NE and DA+NE group were significantly increased (P<0.05). Compared with the LPS group, the serum IL-6 and TNF-k in the NE and DA+NE group decreased to varying degrees (P<0.05). Compared with the NE group, the serum IL-6 and TNF-k levels in the DA+NE group decreased more significantly (P<0.05). Compared with the control group, the expression of NF-kB p65 protein in the LPS, NE and DA+NE group was significantly increased(P＜0.05). Compared with the LPS group, the expressions of NF-kB p65 protein in the NE and DA+NE group were reduced to varying degrees (P<0.05). Compared with the NE group, the expression of NF-kB p65 protein in the DA+NE group decreased more significantly(P<0.05). Dopamine combined with NE has a good protective effect on acute liver injury caused by septic shock in rats. [ABSTRACT FROM AUTHOR]