A Computational Study of the S 2 State in the Oxygen-Evolving Complex of Photosystem II by Electron Paramagnetic Resonance Spectroscopy.

The S2 state produces two basic electron paramagnetic resonance signal types due to the manganese cluster in oxygen-evolving complex, which are influenced by the solvents, and cryoprotectant added to the photosystem II samples. It is presumed that a single manganese center oxidation occurs on S1 → S2 state transition. The S2 state has readily visible multiline and g 4.1 electron paramagnetic resonance signals and hence it has been the most studied of all the Kok cycle intermediates due to the ease of experimental preparation and stability. The S2 state was studied using electron paramagnetic resonance spectroscopy at X-band frequencies. The aim of this study was to determine the spin states of the g 4.1 signal. The multiline signal was observed to arise from a ground state spin ½ centre while the g 4.1 signal generated at ≈140 K NIR illumination was proposed to arise from a spin 5 2 center with rhombic distortion. The 'ground' state g 4.1 signal was generated solely or by conversion from the multiline. The data analysis methods used involved numerical simulations of the experimental spectra on relevant models of the oxygen-evolving complex cluster. A strong focus in this paper was on the 'ground' state g 4.1 signal, whether it is a rhombic 5 2 spin state signal or an axial 3 2 spin state signal. The data supported an X-band CW-EPR-generated g 4.1 signal as originating from a near rhombic spin 5/2 of the S2 state of the PSII manganese cluster. [ABSTRACT FROM AUTHOR]