Bioactive sesterterpenoids from the fungus Penicillium roqueforti YJ-14.

Seven previously undescribed sesterterpenes were characterized from Penicillium roqueforti YJ-14 by solid fermentation. Their structures were initially investigated in detail by spectroscopic analyses and HR-ESI-MS and were further confirmed by X-crystallography. In in vitro bioassays, compounds 1 , 5 and 7 showed cytotoxic activity against the MCF-7 breast cancer cell line with IC 50 values of 7.98 ± 0.93, 6.42 ± 0.41 and 7.32 ± 0.18 μ M, respectively. Compounds 5 and 7 displayed significant cytotoxicity against the A549 lung cancer cell line (IC 50 values of 4.83 ± 0.22 μ M and 4.58 ± 0.85 μ M, respectively). In addition, compound 5 showed an obvious inhibitory effect on nitric oxide production in LPS-activated RAW264.7 macrophages with an IC 50 value of 9.53 ± 0.16 μ M. [Display omitted] • Seven new sesterterpenes were isolated from Penicillium roqueforti YJ-14. • Three compounds showed cytotoxic activity. • One compound showed anti-inflammatory activity. [ABSTRACT FROM AUTHOR]