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Immortalization of shrimp lymphoid cells by hybridizing with the continuous cell line Sf9 leading to the development of 'PmLyO-Sf9 '.
- Source :
-
Fish & Shellfish Immunology . Jun2021, Vol. 113, p196-207. 12p. - Publication Year :
- 2021
-
Abstract
- Shrimp progressively gets more attention among marine invertebrates from researchers all over the world due to it being a healthy food as well as having economic importance. There were a lot of attempts to develop a continuous cell line from shrimp but none successful. In this context a novel hybrid cell line named ' Pm LyO- Sf 9' could be developed by fusing shrimp lymphoid organ cells with Sf 9 cells after to metabolic blocking of Sf 9 cells using puromycin and actinomycin D and effecting the fusion by way of PEG application. The cells are maintained and multiplied in a mixture of SCCM and TNM-FH having osmolality 550 mOsm kg−1 and pH 6.8. Transmission electron microscopy of the hybrid cells revealed the presence of two nuclei during the initial stages and a single nucleus subsequently. The cell line is with shrimp and Sf 9 genomic components and shrimp specific protein and is susceptible to WSSV. Shrimp elongation factor, Sf9 beta-actin, shrimp STAT and peroxinectin could be expresses through RT-PCR in the cell line. This is the first successful report of a hybrid cell line with shrimp genomic components and envisaged to be recognized a model system for multitudes of biomedical research in vitro. The cell line is in the National Cell Line Repository of ICAR – National Bureaue of Fish Genetic Resources, Lucknow, India. • A novel hybrid cell line ' Pm LyO- Sf 9' developed by fusing shrimp lymphoid organ cells with Sf 9 cells. • Morphologically, hybrid cells are bi- and tri-polar with tail like extension. • Electron microscopy analysis revealed two distinct nuclei in early stages. • Shrimp and Sf9 genomic components were separately amplified. • Shrimp immune genes peroxinectin and STAT expression confirmed in hybrid cell line. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CELL lines
*SHRIMPS
*MARINE invertebrates
*GERMPLASM
*DACTINOMYCIN
Subjects
Details
- Language :
- English
- ISSN :
- 10504648
- Volume :
- 113
- Database :
- Academic Search Index
- Journal :
- Fish & Shellfish Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 150148010
- Full Text :
- https://doi.org/10.1016/j.fsi.2021.03.023