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The downstream RAF-1 signaling of fibroblast growth factor-23 participates in the osteogenetic effect caused by C-type natriuretic peptide in vitro.
- Source :
-
Advances in Medical Sciences (Elsevier Inc.) . Mar2021, Vol. 66 Issue 1, p206-214. 9p. - Publication Year :
- 2021
-
Abstract
- Several studies have demonstrated that C-type natriuretic peptide (CNP) stimulates osteoblastic proliferation seemly via antagonizing the expression of fibroblast growth factor (FGF)-23 in vitro. The main aim of the present study is to probe whether the post-receptor pathways of FGF-23 participate in osteogenesis caused by CNP. Osteoblasts were cultured in the absence or presence of CNP: 0, 10, and 100 pmol/L, for 24 h, 48 h and 72 h, respectively. The findings of the present study indicated that osteoblastic proliferation was directly promoted by exogenous CNP in a dose-dependent manner; osteoblastic FGF-23 was significantly down-regulated by CNP at 24 h post-treatment; RAF-1, extracellular signal-regulated kinases (ERK), and P38 were substantially suppressed by CNP in a dose- and time-dependent manner; and signal transducer and activator of transcription (STAT)-1 was not changed on the premise of the down-regulated FGF-23 in osteoblasts treated with CNP. CNP may promote osteogenesis via inhibiting ERK and P38, rather than STAT-1, in the downstream of FGF-23/RAF-1 pathway. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 18961126
- Volume :
- 66
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Advances in Medical Sciences (Elsevier Inc.)
- Publication Type :
- Academic Journal
- Accession number :
- 150082757
- Full Text :
- https://doi.org/10.1016/j.advms.2021.03.003