沉默 Pard3 基因对宫颈癌细胞系 SiHa 迁移、 侵袭的影响及其机制.

Objective To observe the effect of silencing partitoning defective gene 3（PARD3）on the migration and invasion of cervical cancer cell line SiHa，and to explore its mechanism. Methods SiHa cells were divided into the siR⁃ NA1 group，siRNA2 group，and siRNC group. SiHa cells in the siRNA1 group were transfected with PARD3 small inter⁃ ference RNA1（siRNA1），the siRNA2 group with PARD3 small interference RNA2（siRNA2），and the siRNC group with blank vector，and then they were incubated for 48 h after transfection. Transwell migration and invasion assay was used to observe the migration and invasion abilities of the three groups，which were represented by the relative number of migration cells and the relative number of invasion cells，respectively. Pard3，JAK2，STAT3，E-cadherin and Vimentin mRNAs were detected by real-time quantitative PCR. Pard3，JAK2，STAT3，E-cadherin，Vimentin，p-JAK2，and p-STAT3 pro⁃ teins in the three groups were detected by Western blotting. Results The relative number of migration cells and the rela⁃ tive number of invasion cells in the siRNA1 and siRNA2 groups were higher than those in the siRNC group（all P<0. 05）. The relative expression levels of PARD3，E-cadherin mRNA and protein in the siRNA1 and siRNA2 groups were lower than those in the siRNC group（all P<0. 05），and the relative expression levels of Vimentin mRNA，Vimentin protein，pJAK2 protein and p-STAT3 protein in the siRNA1 and siRNA2 groups were higher than those in the siRNC group（all P< 0. 05）. Conclusion Silencing PARD3 gene can promote the migration and invasion of SiHa cells，and the mechanism may be related to the regulation of JAK2/STAT3 phosphorylation signal transduction pathway. [ABSTRACT FROM AUTHOR]