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C-type lectin with a QPN motif from swimming crab Portunus trituberculatus displays broad nonself-recognition ability and functions as an opsonin.
- Source :
-
Developmental & Comparative Immunology . Jul2021, Vol. 120, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
-
Abstract
- In the immune system, C-type lectins, as pattern recognition receptors, have an important function. Carbohydrate-recognition domains (CRDs) endow C-type lectins with the function of recognizing and scavenging non-self factors. In the present study, a new C-type lectin (designated as PtCTL-9 according to the order of discovery) from swimming crab (Portunus trituberculatus) was characterized. QPN (Gln-Pro-Asn) and FHS (Phe-His-Ser) were identified as the key motifs that determine carbohydrate binding. Motif QPN was mutated to QPD (Gln-Pro-Asp) (M1) and EPN (Glu-Pro-Asn) (M2) to study its immune function and for comparative analysis. The results showed that PtCTL-9 displayed broad non-self immunity. PtCTL-9 could also function as an opsonin to promote phagocytosis and the in vitro encapsulation of hemocytes. These results indicated that PtCTL-9 has an extensive nonself-recognition ability, regulates pathogen clearance, and its QPN motif is important in PtCTL-9's immune function. • A C-type lectin PtCTL-9 with a novel QPN motif was identified from Portunus trituberculatus. • PtCTL-9 possessed strong binding activity to nonself particles while its two mutants displayed weak binding activity. • PtCTL-9 inhibited the growth of S. cerevisiae. • PtCTL-9 promoted phagocytosis and encapsulation activity of hemocytes in vitro. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0145305X
- Volume :
- 120
- Database :
- Academic Search Index
- Journal :
- Developmental & Comparative Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 149734837
- Full Text :
- https://doi.org/10.1016/j.dci.2021.104066