Covalent modification by phenolic extract improves the structural properties and antioxidant activities of the protein isolate from Cinnamomum camphora seed kernel.

• Covalent modification of PI by PPE at different concentrations was investigated. • A total of 15 bioactive compounds in PPE were tentatively identified by UPLC-ESI-MS n. • Secondary and tertiary structures of PI were changed by the formation of PI-PPE complexes. • PI-PPE complexes exhibited stronger thermal stability and antioxidant activities than those of PI. In this study, protein isolate (PI) and purified phenolic extract (PPE) were prepared from Cinnamomum camphora seed kernel (CCSK). The effects of covalent modification of PI by PPE at different concentrations (1, 2, 3, 4 and 5%, w/w) were investigated with respect to structural properties and antioxidant activities of protein. Fifteen bioactive compounds in PPE were tentatively identified by UPLC-ESI-MS n. With the increase of PPE concentration, the turbidity, covalent binding rate, phenolic content and color intensity of the PI-PPE complexes were gradually increased. Fourier transform infrared spectroscopy and circular dichroism spectroscopy analysis showed that the secondary and tertiary structures of the complexes were changed and became greater order than PI. Furthermore, the complexes exhibited stronger thermal stability and antioxidant activities than those of PI. These results suggested that the protein–phenolic covalent complexes obtained from CCSK may have great potential to be used in food formulations as functional ingredients. [ABSTRACT FROM AUTHOR]