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Perfluorocarbon Protects against Lipopolysaccharide-Induced Apoptosis of Endothelial Cells in Pulmonary Microvessels.
- Source :
-
Bulletin of Experimental Biology & Medicine . Feb2021, Vol. 170 Issue 4, p410-414. 5p. - Publication Year :
- 2021
-
Abstract
- This study was aimed to explore the effect and mechanisms of action of perfluorocarbon on LPS-induced apoptosis of pulmonary microvascular endothelial cells (PMVEC) isolated from Sprague-Dawley rats. Apoptosis rates were assessed by flow cytometry. Ultrastructural characteristics of PMVEC were evaluated by transmission electron microscopy. The protein expression of cleaved caspase-3 was measured using Western blotting. LPS significantly increased the level of apoptosis, induced the appearance of ultrastructural changes typical of apoptosis, up-regulated the expression of active caspase-3 protein. These effects of LPS were attenuated by co-administration of perfluorocarbon. These results suggest that perfluorocarbon can attenuate LPS-induced apoptosis of PMVEC by inhibiting TLR-4 signaling and caspase-3 activation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00074888
- Volume :
- 170
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Bulletin of Experimental Biology & Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 149419864
- Full Text :
- https://doi.org/10.1007/s10517-021-05077-8