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Hapten synthesis and antibody production for the development of a paper immunosensor for lean meat powder zilpaterol.
- Source :
-
New Journal of Chemistry . 3/21/2021, Vol. 45 Issue 11, p5228-5239. 12p. - Publication Year :
- 2021
-
Abstract
- In this study, we describe the design and successful synthesis of a new hapten, which we used to prepare an anti-zilpaterol mouse monoclonal antibody (mAb) with an IC50 of 0.31 ng mL−1 and a limit of detection (LOD) of 0.02 ng mL−1. A colloidal gold immunochromatographic test strip was further developed and optimized using the antibody, and cross-reactivity experiments were carried out to prove its good specificity. Semi-quantitative and quantitative detections of zilpaterol were evaluated in swine urine and pork samples using the test strips. For semi-quantitative detection in swine urine and pork samples, the visual limit of detection values were 0.5 μg kg−1 and 0.5 μg kg−1, and the cut-off values were 5 μg kg−1 and 5 μg kg−1, respectively. For quantitative detection, the calculated limit of detection values were 0.08 μg kg−1 and 0.12 μg kg−1, and the remaining values were 10 μg kg−1 and 10 μg kg−1. The developed gold nanoparticle-based immunochromatographic assay is highly suitable for on-site detection and rapid preliminary screening of zilpaterol in swine urine and pork samples. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ANTIBODY formation
*COLLOIDAL gold
*DETECTION limit
*MONOCLONAL antibodies
*POWDERS
Subjects
Details
- Language :
- English
- ISSN :
- 11440546
- Volume :
- 45
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- New Journal of Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 149410494
- Full Text :
- https://doi.org/10.1039/d1nj00426c