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Insights into the genome and secretome of Fusarium metavorans DSM105788 by cultivation on agro-residual biomass and synthetic nutrient sources.

Authors :
Brandt, Sophie C.
Brognaro, Hévila
Ali, Arslan
Ellinger, Bernhard
Maibach, Katharina
Rühl, Martin
Wrenger, Carsten
Schlüter, Hartmut
Schäfer, Wilhelm
Betzel, Christian
Janssen, Stefan
Gand, Martin
Source :
Biotechnology for Biofuels. 3/20/2021, Vol. 14 Issue 1, p1-22. 22p.
Publication Year :
2021

Abstract

Background: The transition to a biobased economy involving the depolymerization and fermentation of renewable agro-industrial sources is a challenge that can only be met by achieving the efficient hydrolysis of biomass to monosaccharides. In nature, lignocellulosic biomass is mainly decomposed by fungi. We recently identified six efficient cellulose degraders by screening fungi from Vietnam. Results: We characterized a high-performance cellulase-producing strain, with an activity of 0.06 U/mg, which was identified as a member of the Fusarium solani species complex linkage 6 (Fusarium metavorans), isolated from mangrove wood (FW16.1, deposited as DSM105788). The genome, representing nine potential chromosomes, was sequenced using PacBio and Illumina technology. In-depth secretome analysis using six different synthetic and artificial cellulose substrates and two agro-industrial waste products identified 500 proteins, including 135 enzymes assigned to five different carbohydrate-active enzyme (CAZyme) classes. The F. metavorans enzyme cocktail was tested for saccharification activity on pre-treated sugarcane bagasse, as well as untreated sugarcane bagasse and maize leaves, where it was complemented with the commercial enzyme mixture Accellerase 1500. In the untreated sugarcane bagasse and maize leaves, initial cell wall degradation was observed in the presence of at least 196 µg/mL of the in-house cocktail. Increasing the dose to 336 µg/mL facilitated the saccharification of untreated sugarcane biomass, but had no further effect on the pre-treated biomass. Conclusion: Our results show that F. metavorans DSM105788 is a promising alternative pre-treatment for the degradation of agro-industrial lignocellulosic materials. The enzyme cocktail promotes the debranching of biopolymers surrounding the cellulose fibers and releases reduced sugars without process disadvantages or loss of carbohydrates. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
17546834
Volume :
14
Issue :
1
Database :
Academic Search Index
Journal :
Biotechnology for Biofuels
Publication Type :
Academic Journal
Accession number :
149397980
Full Text :
https://doi.org/10.1186/s13068-021-01927-9