Padronização de um protocolo para assepsia de segmentos nodais de Phalaenopsis para clonagem in vitro.

Plant biotechnology san área of high importance since it has for obtaining plant organisms with characteristics superior to those already on the market. Cloning is one of the tolos that for this function, through it, organisms with characteristics of interest are selected and this individual is multiplied, ensuring that there generated plants are genetically identical to the desired matrix, establishing a standardization. Knowing that the ornamental plants sector contributes significantly to the economy and among the most esteemed ornamental plants among Brazilians, there are orchids which have acquired cultural visibility and a large number of collectors in recent years. The objective of the present work was to establish an efficient assepsis protocol for lateral meristems and to obtain clones of the orchid of the genus Phalaenopsis. For methodology, aiming at the standardization o fan asepsis protocol, 4 treatments were developed and tested with different combinations (concentration x time) of agents such as sodium hypochlorite, alcohol, copper, tween and washing the explants with steriledistilled wáter for meristems of the orchid of the genus Phalaenopsis. The meristems, also known as lateral bud, were removed from the stem of the seedlings, from their floral stems. As forobtained clones, the experiment carried out consisted of inoculating the meristems, after an asepsis process, in Knudson culture media, containing different combinations of grow thregulators, using anauxin and cytokinin. The results obtained show that, no asepsis treatments tested in this work, presented significant and positive results, so it was not posible to establish, as yet, an asepsis protocol for lateral meristem of orchids of the genus Phalaenopsis. Since all meristems suffered contamination and oxidation. There for, it was not yet posible to obtain clones, using this methodology. This work serves as aninitialbasis, for future research, regarding cloning through meristems in Phalaenopsis. [ABSTRACT FROM AUTHOR]