High‐oleic acid content, nontransgenic allotetraploid cotton (Gossypium hirsutum L.) generated by knockout of GhFAD2 genes with CRISPR/Cas9 system.

High-oleic acid content, nontransgenic allotetraploid cotton (Gossypium hirsutum L.) generated by knockout of GhFAD2 genes with CRISPR/Cas9 system Recently, the availability of versatile CRISPR/Cas genome editing techniques has allowed scientists to precisely edit the expressions of target genes without T-DNA insertions (Wang I et al i ., 2020; Zhang I et al i ., 2020). Keywords: cotton (Gossypium hirsutum); genome editing; CRISPR/Cas9; high-oleic seeds; nontransgenic EN cotton (Gossypium hirsutum) genome editing CRISPR/Cas9 high-oleic seeds nontransgenic 424 426 3 03/15/21 20210301 NES 210301 Cotton ( I Gossypium hirsutum i ), the most important cash crop for natural textile fibres, meanwhile, represents the fifth largest source of vegetable oil for human consumption in the world. These complex editing patterns indicated that the Cas9 was active in T1 generation or that the T0 plants contained chimeric mutations, which is consistent with the gene-editing profile of I BnITPK i genes in I Brassica napus i (Sashidhar I et al i ., 2020). [Extracted from the article]