Impact of winterization on fatty acids' composition, isomers, and oxidative stability of conjugated linoleic acids produced from selected vegetable oils.

Degummed, refined, winterized (at −60°C), and non‐winterized soybean, sunflower, cottonseed, and safflower oil samples were used for the production of conjugated linoleic acid (CLA) by urea treatment. Fatty acids' composition and CLA isomers of winterized and non‐winterized versions of oil were analyzed on GC‐MS. Winterization improved the concentration of linoleic acids (LAs) in all vegetable oils. Safflower oil exhibited the highest concentration of LAs at 84.19% followed by sunflower oil (68.54%), cottonseed oil (63.74%), and soybean oil (59.88%), respectively. In safflower oil derived CLAs, concentrations of ∆9c,11t‐18:2, ∆10t,12c‐18:2, ∆9c,11c‐18:2, ∆10c,12t‐18:2, ∆8,9,11,10,12c‐c18:2, and ∆8,9,11,10,12t‐t18:2 were calculated 47.68%, 39.95%, 5.42%, 1.89%, 3.06%, 0.55%, and 1.45%, while in winterized safflower oil derived CLAs, these concentrations were 55.67%, 41.92%, 0.19%, 0.78%, 0.65%, 0.47%, and 0.32%, respectively, with significant difference in induction period and peroxide value. Yield of CLA from winterize soybean, sunflower cottonseed, and safflower oil was 22.58%, 24.95%, 23.16%, and 36.68%. Practical applications: Conjugated linoleic acids (CLAs) are naturally present in milk, dairy products, and beef. However, the amount of CLAs in dairy products and beef accounts limited moreover, the yield of CLA supplement produced from safflower oil is about 20%. To make the industrial processes economical, and sustainable fortification strategies, yield of CLA supplement should be increased. In the current study, winterized version of sunflower, soybean, cottonseed, and safflower oils were used to produce CLA supplement. Using winterized version, the yield of CLA supplement from safflower oil was increased to 36.68%. This work is highly suitable for the food industries to produce functional foods containing CLA. [ABSTRACT FROM AUTHOR]