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Pseudorabies virus UL24 antagonizes OASL-mediated antiviral effect.
- Source :
-
Virus Research . Apr2021, Vol. 295, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
-
Abstract
- • PRV infection leads to endogenous OASL downregulation. • RIG-I is required for OASL-mediated anti-PRV activity. • OASL exerts inhibitory effects on PRV proliferation. • PRV UL24 impairs RIG-I signaling. • PRV UL24 reduces OASL transcription in an IRF3-dependent manner. Oligoadenylate synthetases-like (OASL) protein exerts various effects on DNA and RNA viruses by inhibiting cGAS-mediated IFN production and by enhancing RIG-I-mediated IFN induction, respectively. In this study, we aimed to examine the role of OASL in pseudorabies virus (PRV) proliferation and investigate the function of the PRV UL24 protein in cellular innate immunity. We found that OASL regulates PRV proliferation by enhancing RIG-I signaling. PRV infection decreased the expression of OASL at both the mRNA and protein levels in PK15 and HeLa cells. OASL expression suppressed the proliferation of PRV in a RIG-I-dependent manner and boosted RIG-I-mediated IFN expression as well as IFN-stimulated gene (ISG) induction. In contrast, knockdown of OASL enhanced PRV proliferation and reduced RIG-I signaling. However, the PRV UL24 protein was found to impair RIG-I signaling, thus inhibiting transcription of IFN and ISGs. In addition, the UL24 protein reduced RIG-I-induced expression of endogenous OASL in an IRF3-dependent manner, thereby antagonizing the OASL antiviral effect. Taken together, our findings characterize the role of OASL in PRV proliferation and provide new insights into the role of UL24 in PRV pathogenesis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01681702
- Volume :
- 295
- Database :
- Academic Search Index
- Journal :
- Virus Research
- Publication Type :
- Academic Journal
- Accession number :
- 149125816
- Full Text :
- https://doi.org/10.1016/j.virusres.2020.198276