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Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates.

Authors :
Gómez-Molero, Emilia
De-la-Pinta, Iker
Fernández-Pereira, Jordan
Groß, Uwe
Weig, Michael
Quindós, Guillermo
de Groot, Piet W. J.
Bader, Oliver
Source :
Journal of Fungi. Jan2021, Vol. 7 Issue 1, p1-12. 12p.
Publication Year :
2021

Abstract

Candida parapsilosis is a frequent cause of fungal bloodstream infections, especially in critically ill neonates or immunocompromised patients. Due to the formation of biofilms, the use of indwelling catheters and other medical devices increases the risk of infection and complicates treatment, as cells embedded in biofilms display reduced drug susceptibility. Therefore, biofilm formation may be a significant clinical parameter, guiding downstream therapeutic choices. Here, we phenotypically characterized 120 selected isolates out of a prospective collection of 215 clinical C. parapsilosis isolates, determining biofilm formation, major emerging colony morphotype, and antifungal drug susceptibility of the isolates and their biofilms. In our isolate set, increased biofilm formation capacity was independent of body site of isolation and not predictable using standard or modified European Committee on Antimicrobial Susceptibility Testing (EUCAST) drug susceptibility testing protocols. In contrast, biofilm formation was strongly correlated with the appearance of non-smooth colony morphotypes and invasiveness into agar plates. Our data suggest that the observation of non-smooth colony morphotypes in cultures of C. parapsilosis may help as an indicator to consider the initiation of anti-biofilm-active therapy, such as the switch from azole- to echinocandinor polyene-based strategies, especially in case of infections by potent biofilm-forming strains. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
2309608X
Volume :
7
Issue :
1
Database :
Academic Search Index
Journal :
Journal of Fungi
Publication Type :
Academic Journal
Accession number :
149098394
Full Text :
https://doi.org/10.3390/jof7010033