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A colorimetric paper-based ATONP-ALP nanobiosensor for selective detection of Cd2+ ions in clams and mussels.
- Source :
-
Analytical & Bioanalytical Chemistry . Mar2021, Vol. 413 Issue 6, p1715-1727. 13p. - Publication Year :
- 2021
-
Abstract
- A colorimetric paper-based enzyme-coupled antimony tin oxide nanoparticle (ATONP) nanobiosensor for selective detection of Cd2+ ions in clams and mussels is presented. Alkaline phosphatase (ALP) was immobilized on ATONPs via 16-phosphonohexadecanoic acid (16-PHA) to develop ATONP-ALP nanobiosensor. The biosensor was characterized using XPS, Raman spectroscopy, SEM, and EDX. ATONP-ALP nanobiosensor exhibited high selectivity towards detection of Cd2+ ion with a LOD 0.006 μg L−1 and linear range of detection 0.005–1 μg L−1. The developed biosensor was further integrated into a low-cost paper-based format. A visual color change was obtained for Cd2+ ion in the range 0.1–10 μg L−1. The developed biosensor was successfully demonstrated for the analysis of Cd2+ ions in clams with recoveries 101–104%. The ATONP-ALP nanobiosensor was validated using mussel tissue (BCR-668) and the conventional ICP-OES and ICP-MS techniques. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CLAMS
*MUSSELS
*IONS
*ION analysis
*TIN oxides
*ALKALINE phosphatase
*COLORIMETRY
Subjects
Details
- Language :
- English
- ISSN :
- 16182642
- Volume :
- 413
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Analytical & Bioanalytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 149031213
- Full Text :
- https://doi.org/10.1007/s00216-020-03140-3