Temporary addition of carbon fibers facilitates methanogenic degradation of ethanol during anaerobic treatment.

Syntrophic methanogenesis can be improved by the addition of conductive materials. In this study, conductive carbon fibers (CFs) were applied to efficiently enrich syntrophic microorganisms with potential direct interspecies electron transfer (DIET) ability and promote methanogenic activity. With ethanol as the substrate, CFs shortened the acclimation time remarkably. The maximum methane production rate and the ethanol degradation rate of suspended biomass were increased by 40% and 68%, respectively, even when CFs were subsequently removed. However, with acetate and propionate as the mixed substrate, CFs decreased the methanogenic activity. In the reactor fed with ethanol, CFs increased the relative abundance of Geobacter , Desulfovibrio , and methanogens by 57%, 39%, and 63%, respectively. Methanosaeta possessed most methane production genes and might involve in DIET. Furthermore, CFs increased the relative abundance of ethanol-degradation genes assigned to Geobacter , Desulfovibrio and Pelobacter , suggesting the promoted ethanol-degradation. The triggered electron transport system activity and acetoclastic methanogenesis also explained the accelerated effects on ethanol-degradation by long-term acclimation with CFs. Notably, the dominance of Geobacter and Methanosaeta combined with the increased electron transfer constant in the CFs-amended ethanol reactor indicated the potential role of DIET after the removal of CFs, which deserved further clarification. Unlabelled Image • Carbon fibers could enrich ethanol-oxidizing Geobacter and Desulfovibrio. • Methanogenesis from ethanol was improved even when carbon fibers were removed. • Acetoclastic methanogenesis was triggered by carbon fibers in ethanol reactors. • The promotion of carbon fibers was strongly affected by organic substrates. [ABSTRACT FROM AUTHOR]