Bacillus licheniformis VO1’den α-Amilaz Üretimi için Tarımsal Endüstriyel Atıkların ve Fiziksel Faktörlerin İncelenmesi.

Evaluation of biomass is of great interest in the context of preventing environmental pollution. Agricultural industry wastes and by-products occur in large amounts and cause environmental pollution when left to decay. The use of agro-industrial wastes in solid state fermentation (SSF) technology offers several advantages in the production of antibiotics, pigments, flavors and industrially important enzymes such as amylases, proteases, lipases, cellulases and lipases. α-Amylase is an enzyme that catalyzes the random hydrolysis of starch. These enzymes are used in various biotechnological processes such as textile, paper, food, biofuels, detergents and pharmaceutical industries. In this study, the selected bacterial strain, Bacillus licheniformis VO1, was inoculated into solid state fermentation media containing apple, melon, banana and orange peels, and the highest α-amylase production was obtained from the apple peel medium among the tested solid substrates. Fermentation time, fermentation temperature, initial pH, inoculum size, the effects of nitrogen, carbon and metal sources were examined separately. Maximum α-amylase production was achieved at 45 °C, pH 6.0 and 48 hours. When the effect of various carbon and nitrogen sources added to the fermentation medium where apple peels are examined, the maximum α- amylase production was obtained from the media with starch and yeast extract, respectively. Considering the results obtained, the production of α-amylase from Bacillus licheniformis VO1 can be considered as a potential candidate for future environmental improvement processes, with the use of wastes such as apple peels in the fruit processing industry as a solid state fermentation (SSF). [ABSTRACT FROM AUTHOR]