Horseradish Peroksidaz Nano Biyokatalizörü İle Hidrokinon’un Polimerleştirilmesi.

In this study, the effects of flower shaped hybrid nanobiocatalyst (HRP-Fe2+) containing horseradish peroxidase (HRP) enzyme and Fe2+ ions on the polymerization of hydroquinone were investigated. According to the obtained results, HRP-Fe2+ hybrid nanobiocatalyst in the presence of hydrogen peroxide (H2O2) initiator has shown enhanced catalytic stability at high reaction temperatures compared to that of free HRP enzyme. Poly(hydroquinone) was successfully synthesized with 44% of yield in pH 7.4 buffer at 60 oC reaction temperature with 5 weight % HRP-Fe2+catalyst loading. HRP-Fe2+ nanobiocatalyst also showed some degree of catalytic activity even at 70 oC reaction temperature without having denaturation, in which free HRP enzyme denatures. On the other hand, HRP-Fe2+ also showed lower catalytic activity in the polymerization of hydroquinone compared to that of the free HRP enzyme. It was observed that both polymerizations resulted in polymer product with almost the same yields, but the polymers obtained from using the free HRP enzyme had higher molecular weights in contrast with the polymers obtained from HRP-Fe2+ biocatalyst. It was concluded that the catalytic activity of the HRP-Fe2+ nanobiocatalyst slightly decreased compared to the free HRP enzyme. [ABSTRACT FROM AUTHOR]