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Regulation of circular RNAs act as ceRNA in a hypoxic pulmonary hypertension rat model.
- Source :
-
Genomics . Jan2021:Part 1, Vol. 113 Issue 1, p11-19. 9p. - Publication Year :
- 2021
-
Abstract
- To explore potential critical genes and identify circular RNAs (circRNAs) that act as the competitive endogenous RNA (ceRNA) in a hypoxic pulmonary hypertension (HPH) rat model. Constructed rat model, and a bioinformatics method was used to analyse differentially expressed (DE) genes and construct a circRNA-miRNA-mRNA ceRNA regulatory network. Then, qRT-PCR was used to verify. The significant DEcircRNAs/DEmiRNAs/DEmRNAs was showed, and a ceRNA network with 8 DEcircRNAs, 9 DEmiRNAs and 46 DEmRNAs were constructed. The functional enrichment suggested the inflammatory response, NF-κB signalling, MAPK cascade and Toll-like receptor were associated with HPH. Further assessment confirmed that circ_002723, circ_008021, circ_016925 and circ_020581 could have a potential ceRNA mechanism by sponging miR-23a or miR-21 to control downstream target gene and be involved in the pathophysiology of HPH. The qRT-PCR validation results were consistent with the RNA-Seq results. This study revealed potentially important genes, pathways and ceRNA regulatory networks in HPH. • The HPH rat model was successfully constructed. • Dysregulated circRNAs/miRNAs/mRNAs of HPH from rat lung tissues were integrated, and a circRNA-miRNA-mRNA ceRNA regulatory network was constructed. • A variety of novel genes (including circRNAs/miRNAs/mRNAs) and pathways (including KEGG pathway and ceRNA network) were possibly involved in the occurrence and development of HPH. • The qRT-PCR validation results were consistent with the RNA-Seq results. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PULMONARY hypertension
*RATS
*TOLL-like receptors
*CIRCULAR RNA
*INFLAMMATION
*RNA
Subjects
Details
- Language :
- English
- ISSN :
- 08887543
- Volume :
- 113
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Genomics
- Publication Type :
- Academic Journal
- Accession number :
- 148234582
- Full Text :
- https://doi.org/10.1016/j.ygeno.2020.11.021