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miR-31-5p promotes proliferation and inhibits apoptosis of goat hair follicle stem cells by targeting RASA1/MAP3K1 pathway.
- Source :
-
Experimental Cell Research . Jan2021, Vol. 398 Issue 2, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
-
Abstract
- The Yangtze River Delta white goat is a sole goat species that can naturally produce superior-quality brush hair. It's worth to mention that study the developmental mechanism of goat hair follicle stem cells is vital for future breed preservation and molecular breeding. In this study, we successfully isolated hair follicle stem cells from the skin tissue of fetal sheep neck spine, and harvested superior-quality and normal-quality brush hair goat tissue. The expression of miR-31-5p in goat hair follicle stem cells was verified by qPCR and Western blot. The effects of overexpression or inhibition of miR-31-5p on the proliferation and apoptosis of hair follicle stem cells were detected by EdU, CCK-8, flow cytometry, etc. miR-31-5p can significantly improve cell proliferation and inhibit cell apoptosis by targeting RASA1 and upregulating MAP3K1 level, whereas miR-31-5p knockdown led to an opposite effect. These results reveal a miR-31-5p-associated regulatory network between miR-31-5p and RASA1/MAP3K1 during the progression of superiorquality brush hair traits. • MAP3K1 was upregulated in hair follicle stem cells in response to overexpression miR-31–5p. • We confirmed RASA1 as a target gene of miR-31–5p. • Inhibition of miR-31–5p promotes apoptosis. • The miR-31–5p/RASA1/MAP3K1 axis regulates hair follicle stem cells activity via MAPK pathway. [ABSTRACT FROM AUTHOR]
- Subjects :
- *STEM cells
*HAIR follicles
*GOATS
*HAIRBRUSHES
*APOPTOSIS
*FETAL tissues
Subjects
Details
- Language :
- English
- ISSN :
- 00144827
- Volume :
- 398
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Experimental Cell Research
- Publication Type :
- Academic Journal
- Accession number :
- 147855255
- Full Text :
- https://doi.org/10.1016/j.yexcr.2020.112441