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Neutralization and binding activity of a human single-chain antibody to ricin toxin.
- Source :
-
Food & Agricultural Immunology . Dec2020, Vol. 31 Issue 1, p63-74. 12p. - Publication Year :
- 2020
-
Abstract
- Ricin toxin (RT), extracted from castor bean, is a type II ribosome-inactivating protein (RIP). As a biothreat agent, there is no effective antidote for RT to date, but recent advances in antibody research may have meet this need. This study aimed to produce human single-chain antibody variable fragments (HuscFvs) that bind to and interfere with RT activity for further clinical use. The purified RT component was used in phage biopanning to select ricin-bound HuscFv-displayed phage clones from synthetic (Tomlinson I + J) phage display libraries. The selected HuscFv, named JE11, showed an almost 100% protective effect to HeLa cells by mixing with RT at a 1:1000 ratio in vitro. The antibody showed an 83% protection against injection of 2 × LD50 RT dose mice within 72 h. This research has therapeutic potential for the diagnosis and treatment of RT poisoning. [ABSTRACT FROM AUTHOR]
- Subjects :
- *RICIN
*TOXINS
*IMMUNOGLOBULINS
*CASTOR beans
*POISONING
Subjects
Details
- Language :
- English
- ISSN :
- 09540105
- Volume :
- 31
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Food & Agricultural Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 147528157
- Full Text :
- https://doi.org/10.1080/09540105.2019.1698521