补肾活血汤治疗股骨头坏死的分子靶点及机制: 网络药理学及蛋白模块分析.

BACKGROUND: Studies have shown that Bushen Huoxue Decoction has a very active role in treating osteonecrosis of the femoral head (ONFH), but the chemical ingredients contained in the compound are more complicated. The action mechanism and pharmacological mechanism of the compound underlying the treatment of ONFH at the molecular level are not yet clear. Therefore, network pharmacology and protein module analyses are used to explain the potential active ingredients and molecular mechanism of Bushen Huoxue Decoction, and provide a certain theoretical basis for the later development of new drugs and better clinical application in the treatment of ONFH. OBJECTIVE: Through network pharmacology and protein module analysis to explore the molecular targets and mechanisms of Bushen Huoxue Decoction in the treatment of ONFH. METHODS: The compounds and their targets in Carthamus tinctorius, Angelica sinensis, Cistanche deserticola, Cornus officinalis, Chinese wolfberry, dodder, Radix Rehmanniae, Eucommia ulmoides, myrrh, Fructus psoraleae and Fructus psoraleae in Bushen Huoxue Decoction were searched by TCMSP and TCMID databases, and the related targets of ONFH were predicted by GeneCards and CTD databases. The drug-meridian-compound-target network was constructed by Cytoscape3.7.2, the protein-protein interaction network was constructed and analyzed by STRING database, and the key protein modules were analyzed by Metascape database. Gene ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were carried out by using DAVID database. RESULTS AND CONCLUSION: A total of 167 active components and 151 action targets of Bushen Huoxue Decoction were screened, with peroxisome proliferator-activated receptor, vascular endothelial growth factor A, tumor necrosis factor and epidermal growth factor receptor as key targets. There were 8 817 related targets of ONFH, 128 proteins in the protein-protein interaction core network, and 5 protein modules. A total of 289 GO biological processes were obtained, and 110 related signal pathways were obtained by enrichment and screening of KEGG pathway, including estrogen, phosphoinositide-3 kinase/ protein kinase, cyclic adenosine phosphate, Wnt signaling, tumor necrosis factor and mitogen-activated protein kinase. These pathways were associated with the occurrence and development of ONFH. The results show that Bushen Huoxue Decoction may treat ONFH by regulating cell metabolism, proliferation and apoptosis, lipid metabolism and promoting angiogenesis, which is the result of the interaction of multi-components, multi-targets and multi-pathways. [ABSTRACT FROM AUTHOR]