A review of pretreatment and analysis of macrolides in food (Update Since 2010).

• Progress in microextraction technology and online technology are introduced. • The evolution and advance of HRMS analyzers are in detailed. • Advantages and applications of electrochemical methods are presented. • The future perspective for newly emerged eco-friendly methods are discussed. Macrolides are versatile broad-spectrum antibiotics whose activity stems from the presence of a macrolide ring. They are widely used in veterinary medicine to prevent and treat disease. However, because of their improper use and the absence of effective regulation, these compounds pose a threat to human health and the environment. Consequently, simple, quick, economical, and effective techniques are required to analyze macrolides in animal-derived foods, biological samples, and environmental samples. This paper presents a comprehensive overview of the pretreatment and analytical methods used for macrolides in various sample matrices, focusing on the developments since 2010. Pretreatment methods mainly include liquid-liquid extraction, solid-phase extraction, matrix solid-phase dispersion, and microextraction methods. Detection and quantification methods mainly include liquid chromatography (coupled to mass spectrometry or other detectors), electrochemical methods, capillary electrophoresis, and immunoassays. Furthermore, a comparison between the pros and cons of these methods and prospects for future developments are also discussed. [ABSTRACT FROM AUTHOR]