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Cytotoxicity of black phosphorus quantum dots on lung-derived cells and the underlying mechanisms.
- Source :
-
Journal of Hazardous Materials . Jan2021, Vol. 402, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
-
Abstract
- • BP-QDs induce cytotoxicity, oxidativestress and cell cycle arrest in twokinds of human lung epithelial cells. • Inhibition of cellular uptake restoresthe cytotoxicity of BP-QDs. • Oxidative stress contributes to cellcycle arrest and cell damage causedby BP-QDs. Black phosphorus quantum dots (BP-QDs) are a new type of zero-dimensional (0D) nanomaterial that has been widely used due of their superior properties in many biomedical fields, but limited studies have focused on the biocompatibility of BP-QDs, particularly in the respiratory system. In this study, we investigated the potential lung cell toxicity of BP-QDs in vitro. Two human lung-derived cells, A549 and Beas-2B, were treated with 5∼20 μg/mL BP-QDs for 24 h. The results showed that BP-QDs triggered significant lung cell toxicity, including a dose-dependent decrease in cell viability, lactate dehydrogenase (LDH) leakage, cell shape changes, cellular oxidative stress and cell cycle arrest. In addition, pretreatment with the classical phagocytosis inhibitor cytochalasin D (Cyto D) alleviated the decrease in cell viability and LDH leakage induced by BP-QDs. In contrast, BP-QDs induced the production of cellular reactive oxygen species (ROS) and decreases in the glutathione level, whereas the ROS scavenger N-acetyl-L-cysteine (NAC) could protect A549 and Beas-2B cells from BP-QD-induced cellular oxidative stress. Taken together, the results from this study indicate that the potential toxic effects and mechanisms of BP-QDs in two different human lung cells should be considered to evaluate the lung cell safety of BP-QDs. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03043894
- Volume :
- 402
- Database :
- Academic Search Index
- Journal :
- Journal of Hazardous Materials
- Publication Type :
- Academic Journal
- Accession number :
- 147070414
- Full Text :
- https://doi.org/10.1016/j.jhazmat.2020.122875