Self-standardization of quality of bacterial cellulose produced by Medusomyces gisevii in nutrient media derived from Miscanthus biomass.

• Biomass of Miscanthus from Western Siberia was pretreated with dilute HNO 3 and NaOH. • BC was synthesized by Medusomyces gisevii under non-sterile conditions. • BC quality does not depend on pretreatment method of Miscanthus biomass. Bacterial cellulose (BC) was synthesized from biomass of Miscanthus grown in West Siberia. Miscanthus biomass was pretreated at atmospheric pressure with 4 wt.% solutions of HNO 3 and NaOH in one and two stages. The effect of four methods of the pretreatment of the feedstock on BC yield and properties was examined. The resultant pulps were subjected to enzymatic hydrolysis with commercial CelloLux-A and BrewZyme BGX enzymes. Biosynthesis of BC was run under static and non-sterile conditions using Medusomyces gisevii Sa-12. The two-stage pretreatment of Miscanthus biomass gave a 20 % increase in BC production compared to the single-stage pretreatment. The resultant BC exhibited a high crystallinity index (88–93 %) and an extraordinarily high content of allomorph Iα (99–100 %), irrespective of the pretreatment method; therefore, it has been revealed for the first time that the Medusomyces gisevii Sa-12 symbiotic culture is capable of self-standardization against the quality of produced BC. [ABSTRACT FROM AUTHOR]