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CircRNA_102179 promotes the proliferation, migration and invasion in non-small cell lung cancer cells by regulating miR-330-5p/HMGB3 axis.
- Source :
-
Pathology - Research & Practice . Nov2020, Vol. 216 Issue 11, pN.PAG-N.PAG. 1p. - Publication Year :
- 2020
-
Abstract
- • CircRNA_102179 expression is significantly up-regulated in NSCLC tissues and cell lines. • 2.CircRNA_102179 promotes NSCLC cell proliferation, migration and invasion. • 3.CircRNA_102179 directly interacted with miR-330−5p. Non-small cell lung cancer (NSCLC) accounting for 85 % of all lung cancer was one of the main causes of death worldwide. In this study, we investigated the role of circRNA_102179 in NSCLC development. The levels of circRNA_102179 in NSCLC tissues and cell lines were determined by quantitative real-time PCR assay (qRT-PCR). CCK8 and colony formation assays were applied to explore the effect of circRNA_102179 on the growth of NSCLC cells in vitro. Transwell assay was utilized to analyze the impact of circRNA_102179 on the migration and invasion of NSCLC cells. Target prediction and luciferase reporter assay were used to identify the interacting miRNA of circRNA_102179. The interaction among circRNA_102179/ miR-330−5p/HMGB3 was further validated by colony formation and Transwell invasion assays. Finally, the mouse xenograft NSCLC model was used to explore the role of circRNA_102179 in the tumor growth of NSCLC cells in vivo. CircRNA_102179 was overexpressed in NSCLC tissues and cells compared with normal lung tissues and human bronchial epithelial cells (HBEs). The down-regulation of circRNA_102179 markedly reduced the proliferation, migration, and invasion of NSCLC cells. Moreover, down-expression of circRNA_102179 significantly increased the level of miR-330−5p/HMGB3 in NSCLC cells. Further functional experiments indicated that over-expression of miR-330−5p reversed the inhibitory effect of circRNA_102179 on NSCLC cells growth, migration, and invasion. Our results reveal that circRNA_102179 facilitates the proliferation, migration, and invasion of NSCLC cell via modulating miR-330−5p/ HMGB3 axis in NSCLC cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- *NON-small-cell lung carcinoma
*CIRCULAR RNA
*CANCER cells
*CELL growth
Subjects
Details
- Language :
- English
- ISSN :
- 03440338
- Volume :
- 216
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Pathology - Research & Practice
- Publication Type :
- Academic Journal
- Accession number :
- 146827905
- Full Text :
- https://doi.org/10.1016/j.prp.2020.153144