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Screening of toll-like receptor signaling pathway-related genes and the response of recombinant glutathione S-transferase A3 protein to thiram induced apoptosis in chicken erythrocytes.
- Source :
-
Developmental & Comparative Immunology . Jan2021, Vol. 114, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
-
Abstract
- The expression of genes related to the Toll-like receptors (TLRs) signaling pathway were determined. Group A, B and C fed with basal diet and group D, E and F induced TD by feeding a basal diet containing 100 mg·kg−1 thiram. rGSTA3 protein was injected at 20 μg·kg−1 in group B, E and at 50 μg·kg−1 in C, F. Results suggested that lameness and death of chondrocytes were significant on day 14. TLRs signaling pathway related genes were screened based on the transcriptome enrichment, and validated on qPCR. IL-7, TLR2, 3, 4, 5, 7, 15, MyD88, MHC-II, MDA5 and TRAF6 were significantly (p < 0.05) expressed in group E and F as compared to group D on day 14 and 23. IL-7, MHCII, TRAF6, TLR3, TLR5, TLR7, and TLR15 determined insignificant in group D compared to group A on day 23. TD occur in an early phase and alleviated in the later period. rGSTA3 protein can prevent apoptosis and repair degraded chondrocytes. Image 1 • TD is an important undetected avian growth plate disease. • Immune genes regulate apoptosis, ultimately death of chondrocytes occured. • Toll-like receptor (TLRs) mediates cellular immune responses to cause apoptosis. • rGSTA3 protein treatment prevents the apoptosis by downregulating TLRs on day 14 and day 23. [ABSTRACT FROM AUTHOR]
- Subjects :
- *TOLL-like receptors
*APOPTOSIS
*GROWTH plate
*PROTEINS
*GENES
Subjects
Details
- Language :
- English
- ISSN :
- 0145305X
- Volume :
- 114
- Database :
- Academic Search Index
- Journal :
- Developmental & Comparative Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 146810585
- Full Text :
- https://doi.org/10.1016/j.dci.2020.103831