Saxitoxin aptasensor based on attenuated internal reflection ellipsometry for seafood.

In this study, we proposed label-free saxitoxin (STX) sensor using STX specific aptamer in combination with spectroscopic ellipsometry (SE) and attenuated internal reflection (AIR) spectroscopic ellipsometry method which is operated under surface plasmon resonance (SPR) conditions. Besides the other surface plasmon resonance-based applications, AIR-SE applications have unique advantages in terms of sensitivity and it was used herein for real-time detection of STX in real samples. Another method, SE, was also used and compared with AIR-SE. Analytical performances were satisfactory with low detection limits and a wide detection range. Limit of detection was 0.01 ng/mL for AIR-SE and 0.11 ng/mL for SE. Both proposed sensors were operable in 0.01 nM–1000 nM STX range. These methods were also used for the accurate, selective, and sensitive detection of STX from fish and shrimp samples. Image 1 • Selective and sensitive detection of saxitoxin from fishery products is reported here. • The first time, a total internal reflection spectrophotometric ellipsometry method is proposed for saxitoxin. • The limit of detection is 0.01 ng/mL which is under the permissible limits (80 ng/mL). [ABSTRACT FROM AUTHOR]