Fabrication and invitro evaluation of electrospun gum ghatti-polyvinyl alcohol polymeric blend green nanofibre mat (GG-PVA NFM) as a novel material for polymeric scaffolds in wound healing.

The present research is mainly based on the fabrication of biodegradable nanofiber mats (NFM) through the process of electrospinning using a novel combination of Gum Ghatti (GG) and Poly vinyl alcohol (PVA). The prepared NFM was crosslinked (CL-1) using Glutaraldeyde-HCl vapours and was characterized for its tensile strength along other analytical characterizations using FTIR, TGA, DSC and XRD. The mechanical strength of the NFM was found to be sufficiently high than in comparison to noncrosslinked sample and PVA NFM. The internal architecture of the CL-1 by use of atomic force microscopy (AFM) revealed that there was very well formed crosslinks suitable for drug loading as well as cell proliferation. The wound healing properties of the CL-1 in mice animal model indicated the healing within 5 days as compared to the control wound. Moreover, the sample was also analysed for its ability as polymeric scaffold and no toxicity was found onto the locally applied tissue on histological investigations. • Preparation of nanofiber mats (NFM) by electrospining technique using a novel combination of Gum Ghatti with Polyvinyl alcohol and later crosslinking with Glutaraldehyde-HCl vapours. • The Surface morphology and internal architecture of the fibers formed were confirmed by SEM and AFM. • Analytical characterizations of CL-1 NFM by using FTIR, TGA, XRD indicated that no incompatibility arose between the two polymer. • The optical contact angle of the crosslinked NFM (CL-1) as well as noncrosslinked NFM (NCL-1) showed less contact angle less than 90° thereby making them both appropriate for tissue growth.. • The mechanical strength of CL-1 was much higher than in comparison to NCL-1 and was selected for tissue growth and wound healing studies and wound healing of the CL-1 NFM was faster than the control wound on mice animal model. [ABSTRACT FROM AUTHOR]