Gama radyasyonun indüklediği nefrotoksiteye karşılık pycnogenolün koruyucu etkisi.

Objective: The radioprotective and antioxidant effect of pycnogenol against gamma radiation-induced nephrotoxicity was investigated at single cell level, tissue level and biochemical parameters in adult male Wistar Albino rats.*Material and Methods: The study was included control group (n=10), antioxidant groups (P37.5, P75, P150, P300, n= 10), only radiation group (y-ışını, n=10) and antioxidant + radiation groups (P37.5+y-ışını, P75+y-ışını, P150+y-ışını, P300+y-ışını, n= 10) on 100 rats. Pycnogenol was dissolved in 5% DMSO. Radiation was applied single fraction with Cobalt-60 teletherapy device. Control and radiation groups were received 5% DMSO. Antioxidant and antioxidant + radiation groups were received pycnogenol at 24, 48 and 72 hours before the irradiation. Twenty-four hours after the administration, the rats were sacrificed with general anesthesia and their kidneys were removed. DNA damage, histopathological evaluation, CAT, SOD activities and MDA levels were evaluated in renal tissue. Results: We were evaluated increased levels of MDA, decreased SOD and CAT activities in the radiation group; however, MDA level was decreased and increased SOD and CAT activities in the antioxidant + radiation group. Comet parameters were increased in the other groups compared to the control group and these parameters were decreased in the antioxidant + radiation groups compared to the radiation group (p <0.001). Histopathologically, impaired histopathology was observed in the other groups compared to the control group and improved histopathology was observed in the antioxidant + radiation groups compared to the radiation group. Conclusion: Pycnogenol is which has been shown to have antioxidant effects, as a protective against kidney damage due to ionized radiation, may be suggested based on the data of our study. However, preclinical and clinical studies are also needed to better understand the mechanism of action and molecular basis and to clarify dose, toxicity and tolerability issues. [ABSTRACT FROM AUTHOR]