恒古骨伤愈合剂对骨质疏松树肾组织 CD34和VEGF的影响.

Objective To evaluate the effect of Osteoking on CD34 and VEGF in kidney of osteoporosis tree shrew. Methods A total of 30 12-month-old female tree shrew （Tupaia belangeri） were randomly divided into control group，model group and treatment group，with 10 in each group. Bilateral ovaries and uterus in the model group and treatment group were removed and osteoporosis was confirmed 6 months later. The treatment group were gavaged with Osteoking once every two days for 3 months，and others were gave with equal volume of saline. Animals were perfused for fixation and collected samples，and IHC mothed was employed to determine the protein expression of CD34 and VEGF in kidney. Results The protein expression of CD34（P < 0.05） and VEGF （P < 0.05） in the model group were significantly higher than the control group after 3 months. Compared with the model group， the protein expression of CD34（P < 0.05） and VEGF（P < 0.05） in the treatment group were significantly decreased. Conclusion Osteoking can effectively protect the kidney by regulating the overexpression of CD34 and VEGF in os-teoporosis castrated tree shrew. [ABSTRACT FROM AUTHOR]