IBS 法在云南人群全同胞鉴定中的应用.

Objective To investigate the practical application of IBS method in the full sibling testing． Methods PowerPlex R 21 System kit was used to amplify the 19 STR loci （D3S1358， D6S1043，D13S317， Penta E，D16S539，D18S51，D2S1338，CSF1PO，Penta D，TH01，vWA，D21S11，D7S820，D5S818， TPOX，D8S1179，D12S391，D19S433，FGA） from the 234 pairs of determined full siblings and 200 unrelated individuals of Han population in Yunnan province，and ABI3130 genetic analyzer automatic detection and Gene Mapper ID v3.2 software were used for STR genotyping．The typing results were calculated on the basis of the IBS method （SF/Z JD0105002-2014） ．Results The rate of full siblings was 82.91% ， and it was higher than 75% in the standard. 17.09% of the samples could not give tendentious opinions. The scores from 13 to 22 had 65 pairs of unrelated individuals， and it accounted for 32.50%.There were 135 pairs of less than 13 points that accounted for 67.50%. Conclusion The application of IBS method in the identification of full siblings in Yunnan has a high value in forensic medicine. [ABSTRACT FROM AUTHOR]