Systematic Chemogenetic Library Assembly.

Chemogenetic libraries, collections of well-defined chemical probes, provide tremendous value to biomedical research but require substantial effort to ensure diversity as well as quality of the contents. We have assembled a chemogenetic library by data mining and crowdsourcing institutional expertise. We are sharing our approach, lessons learned, and disclosing our current collection of 4,185 compounds with their primary annotated gene targets (https://github.com/Novartis/MoaBox). This physical collection is regularly updated and used broadly both within Novartis and in collaboration with external partners. Canham et al. describe their strategy for the systematic assembly of their chemogenetic library known as the Mechanism-of-Action (MoA) Box. Shared are the contents of the library comprising 4,185 chemical probes for over 2,000 mammalian targets and select applications of the MoA Box for drug discovery. [ABSTRACT FROM AUTHOR]