Back to Search
Start Over
Genotyping of Mycobacterium leprae for understanding the distribution and transmission of leprosy in endemic provinces of China.
- Source :
-
International Journal of Infectious Diseases . Sep2020, Vol. 98, p6-13. 8p. - Publication Year :
- 2020
-
Abstract
- • The loci (GTA)9, (AT)17, (AT)15, (TA)18, (TTC)21 and (TA)10 are more highly polymorphic than other loci. • The VNTR profile generated a low-density clustering pattern in the counties of Sichuan and Yunnan. • Clusters were observed from the isolates from Hunan and Guizhou provinces at the county levels. • The most predominantly reported SNP was 3K followed by SNP 1D. • We detected the novel SNP 3J that has not yet been reported in China. • Maximum genotype stability was observed in current and long-standing strains. Understanding the nature of Mycobacterium leprae transmission is vital to implement better control strategies for leprosy elimination. The present study expands the knowledge of county-level strain diversity, distribution, and transmission patterns of leprosy in endemic provinces of China. We genetically characterized 290 clinical isolates of M. leprae from four endemic provinces using variable number tandem repeats (VNTR) and single nucleotide polymorphisms (SNPs). Attained genetic profiles and cluster consequences were contrasted with geographical and migration features of leprosy at county levels. Considering the allelic variability of 17 VNTR loci by the discriminatory index, (GTA)9, (AT)17, (AT)15, (TA)18, (TTC)21, and (TA)10 are reported to be more highly polymorphic than other loci. The VNTR profile generated the low-density clustering pattern in the counties of Sichuan and Yunnan, whereas clusters have been observed from the isolates from Huayuan (N = 6), Yongding (N = 3), Zixing (N = 3), Chenxi (N = 2) and Zhongfang (N = 2) counties of Hunan, and Zhijin (N = 3), Anlong (N = 2), Zhenning (N = 2), and Xixiu (N = 2) counties of Guizhou. In some clusters, people's social relations have been observed between villages. From the 290 clinical isolates, the most predominantly reported SNP was 3K (278, 95.8%), followed by SNP 1D (10, 3.4%), which are typically observed to be predominant in China. We also detected the novel SNP 3J (2, 0.8%), which has not yet been reported in China. The clustering pattern of M. leprae indicates the transmission of leprosy still persists at county levels, suggesting that there is a need to implement better approaches for tracing the close contacts of leprosy patients. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 12019712
- Volume :
- 98
- Database :
- Academic Search Index
- Journal :
- International Journal of Infectious Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 145517905
- Full Text :
- https://doi.org/10.1016/j.ijid.2020.06.032