基因编辑技术在骨关节炎基因治疗中的作用与意义.

BACKGROUND: Osteoarthritis (OA) is a common chronic disease in middle-aged and elderly people with cartilage degeneration injury as the main pathological change. Physiotherapy and drugs can be used to treat OA in the early stage, and surgery can be used in the middle and late stage, but the overall treatment effect is poor. OBJECTIVE: To review the latest development of gene editing technology in OA gene research worldwide. METHODS: With “Gene editing, Osteoarthritis, The CRISPR/Cas9 technology, Gene therapy, Stem cells” as keywords in English and Chinese, respectively, literatures related to OA gene therapy and gene editing technology were retrieved from PubMed, CNKI and WanFang databases from 2000 to 2020, and summarized and analyzed. RESULTS AND CONCLUSION: A variety of growth factors and regulatory factors are beneficial to promoting the synthesis of cartilage matrix and reducing joint inflammation, thereby providing a seed source for gene targeted modification. Gene editing technology can be used to introduce the target genes into the body by oral, surgical, topical, and articular injection through genetic modification of the congenital embryo or release of biological agents to specific anatomical targets to play the role of gene therapy. New progress has been made in the modification and application of OA disease genes. Gene editing has taken a new direction in its application. However, more animal experiments and clinical trials are needed in the future to study the tissue engineering of genetic modification of OA disease genes. [ABSTRACT FROM AUTHOR]