Back to Search
Start Over
Magnet-actuated droplet microfluidic immunosensor coupled with gel imager for detection of microcystin-LR in aquatic products.
- Source :
-
Talanta . Nov2020, Vol. 219, pN.PAG-N.PAG. 1p. - Publication Year :
- 2020
-
Abstract
- Simplicity, sensitivity and high throughput are the qualities researcher always difficultly pursue in the screening surveillance assay for food contaminants. Herein, coupled with a common gel imager as a signal reader, a novel magnet-actuated droplet microfluidic immunosensor without any complicated manipulation system was successfully fabricated for the convenient and sensitive detection of cyanobacteria toxin microcystin-LR (MC-LR) in aquatic products. The proposed sensor demonstrated three superiorities compared to the extensively used microfluidic systems: 1) simple but effective operation only by magnetic force rather than inconvenient actuating devices; 2) high throughput parallel detection on a 15-channels chip; 3) convenient signal readout on an ordinary laboratorial gel imager. This gel imager-assisted magnet-actuated droplet microfluidic immunosensor exhibited a limit of detection as low as 6.0 × 10−4 μg/L with high specificity. The average recoveries were ranged from 92.0% to 96.6% in spiked fish and prawn samples, and showed a good agreement with confirmation method LC-MS/MS, suggesting the proposed method to be an ideal surveillance assay tool for the MC-LR monitoring in aquatic products. Image 1 • A novel magnet-actuated droplet microfluidic immunosensor allow high throughput parallel detection was developed. • A novel magnet-actuated droplet microfluidic immunosensor allow high throughput parallel detection was developed. • Convenient signal readout on a common laboratorial gel imager. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00399140
- Volume :
- 219
- Database :
- Academic Search Index
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 145408860
- Full Text :
- https://doi.org/10.1016/j.talanta.2020.121329