FINDIĞIN POLİFENOLİK MADDELERİNİN ADSORBAN ÖZELLİKTE KOLON DOLGU MADDESİ KULLANILARAK FRAKSİYONLARINA AYRILMASI VE ELDE EDİLEN FRAKSİYONLARIN KARAKTERİZASYONU.

The aim of this study is fractionation of hazelnut polyphenols and their characterization. For this aim, phenolic compounds were extracted with 80 % (v/v) acetone and loaded to column. Low molecular weight (LMW) and high molecular weight fractions (HMW) were fractionated with ethanol and acetone, respectively. Furthermore, LMW fraction was fractionated into four parts according to peaks observed at UV (280nm). FractionV had the highest contents/activities; total phenolics and condensed tannins contents were 77.9 mg gallic acid equivalent (GAE)/g extract and 227 mg catechin equivalent (CE)/g extract, respectively. Antioxidant activities of FractionV were 0.047 mg/mL IC50 value, 1.442 mmol Trolox equivalent (TE)/g extract, 307 mg FeSO4.7H2O/g extract for DPPH, ABTS and FRAP assays, respectively. As a result, it is seen that further fractionation of LMW is not needed and separation of LMW from HMW fraction is enough. HMW fraction could be utilized as a potential source of polyphenols. [ABSTRACT FROM AUTHOR]