Back to Search
Start Over
Circulating histone signature of human lean metabolic-associated fatty liver disease (MAFLD).
- Source :
-
Clinical Epigenetics . 8/26/2020, Vol. 12 Issue 1, pN.PAG-N.PAG. 1p. - Publication Year :
- 2020
-
Abstract
- Background: Although metabolic associate fatty liver disease (MAFLD) is associated with obesity, it can also occur in lean patients. MAFLD is more aggressive in lean patients compared to obese patients, with a higher risk of mortality. Specific biomarkers to diagnose differentially lean or overweight MAFLD are missing. Histones and nucleosomes are released in the bloodstream upon cell death. Here, we propose a new, fast, imaging and epigenetics based approach to investigate the severity of steatosis in lean MAFLD patients. Results: A total of 53 non-obese patients with histologically confirmed diagnosis of MAFLD were recruited. Twenty patients displayed steatosis grade 1 (0–33%), 24 patients with steatosis grade 2 (34–66%) and 9 patients with steatosis grade 3 (67–100%). The levels of circulating nucleosomes were assayed using enzyme-linked immunosorbent assay, while individual histones or histone dimers were assayed in serum samples by means of a new advanced flow cytometry ImageStream(X)-adapted method. Circulating nucleosome levels associated poorly with MAFLD in the absence of obesity. We implemented successfully a multi-channel flow methodology on ImageStream(X), to image single histone staining (H2A, H2B, H3, H4, macroH2A1.1 and macroH2A1.2). We report here a significant depletion of the levels of histone variants macroH2A1.1 and macroH2A1.2 in the serum of lean MAFLD patients, either individually or in complex with H2B. Conclusions: In summary, we identified a new circulating histone signature able to discriminate the severity of steatosis in individuals with lean MAFLD, using a rapid and non-invasive ImageStream(X)-based imaging technology. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FATTY liver
*ENZYME-linked immunosorbent assay
*HISTONES
*CHROMATIN
Subjects
Details
- Language :
- English
- ISSN :
- 18687075
- Volume :
- 12
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Clinical Epigenetics
- Publication Type :
- Academic Journal
- Accession number :
- 145322079
- Full Text :
- https://doi.org/10.1186/s13148-020-00917-2