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LncRNA PCAT-1 upregulates RAP1A through modulating miR-324-5p and promotes survival in lung cancer.

Authors :
Na Huang
Wenjing Dai
Yunhui Li
Jian Sun
Chunlan Ma
Wancheng Li
Huang, Na
Dai, Wenjing
Li, Yunhui
Sun, Jian
Ma, Chunlan
Li, Wancheng
Source :
Archives of Medical Science. 2020, Vol. 16 Issue 5, p1196-1206. 11p.
Publication Year :
2020

Abstract

<bold>Introduction: </bold>Lung cancer is the malignant tumor with the fastest increase in morbidity and mortality and the greatest threat to human health and life. Long non-coding RNA (lncRNA) is emerging as an important regulator in many cancers. Recently, it was found that lncRNA prostate cancer associated transcript 1 (PCAT-1) was up-regulated in lung cancer, playing oncogenic roles. However, the underlying regulatory mechanism of PCAT-1 remains unknown.<bold>Material and Methods: </bold>The expression levels of PCAT-1 and miR-324-5p were analyzed by real-time PCR, and RAP1A expression was determined by western blotting. RNA pull-down, luciferase and western blotting assays were used to examine the target relationship between PCAT-1 and miR-324-5p or that between miR-324-5p and RAP1A. The functional effects of PCAT-1 and miR-324-5p were examined using cell viability and cell apoptosis assays.<bold>Results: </bold>PCAT-1 overexpression remarkably promoted cell proliferation and suppressed cell apoptosis. Mechanistic investigations demonstrated that PCAT-1 can interact with miR-324-5p and repress its expression, thereby increasing the expression of its target RAP1A. Additionally, rescue experiments revealed that PCAT-1 served as an oncogene partly through sponging miR-324-5p and upregulating RAP1A in lung cancer cells.<bold>Conclusions: </bold>Our findings demonstrate that on account of the dual function of pro-proliferation and anti-apoptosis, PCAT-1/miR-324-5p/RAP1A may be novel candidates for application in the diagnosis, prognosis and therapy of lung cancer. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
17341922
Volume :
16
Issue :
5
Database :
Academic Search Index
Journal :
Archives of Medical Science
Publication Type :
Academic Journal
Accession number :
145272921
Full Text :
https://doi.org/10.5114/aoms.2019.84235