日本落叶松种子园群体遗传多样性评价.

Sixteen expressed sequence tag simple sequence repeat markers were used to analyze and evaluate the genetic diversity of Larix kaempferi in seed orchards, clarify the genetic basis of seed orchards, and provide a basis for the formulation of further breeding strategies and the sustainable development of genetic improvement. The results showed that in the population the average number of effective alleles(Ne) was 2.542, the average observed heterozygosity(Ho) was 0.578, the average expected heterozygosity(He) was 0.528, the average Shannon diversity index was 0.972, the average polymorphic information content index(PIC) was 0.485, and the genetic diversity of L. kaempferi seed orchards was high. Most of the loci in the population were consistent with HWE, and the inbreeding coefficient lines Fis and Fit were both negative. The population showed a slight heterozygote overabundance and no inbreeding recession. The results of genetic distance calculation, molecular variance analysis, as well as principal coordinates analysis indicated that the genetic differentiation between the populations was very small, mainly because of the frequent exchange of introduced species in different cultivation areas in China. [ABSTRACT FROM AUTHOR]