Biofilm-producing organisms and their antibiotic sensitivity in chronic otitis media – Mucosal disease.

Objectives: The objective of the study is to know the presence of biofilm-producing organisms in the middle ear mucosa in chronic otitis media (COM) mucosal disease and their antibiotic sensitivity. Materials and Methods: This was a prospective comparative study conducted on 100 patients who underwent tympanoplasty. They were divided into two groups: Group 1 included mucosal active disease (50) and Group 2 included mucosal inactive (50). During surgery, a biopsy from the middle ear mucosa was taken and sent for culture and sensitivity and tested for their biofilm-forming capability using tissue culture plate method. Results: In Group 1, 17 cultures were biofilm-producing organisms and 14 were non/weak biofilm-producing organisms. In Group 2, 14 were biofilm producing and 8 were non/weak biofilm producing. Piperacillin-tazobactam was the most sensitive drug for Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus epidermidis irrespective of their biofilm-producing capability. Linezolid had showed 100% sensitivity for Staphylococcus aureus. Ciprofloxacin was the most resistant drug irrespective of organisms and their biofilm-forming capability.Conclusion: P. aeruginosa and S. aureus are the common organisms present in the mucosa of the middle ear in COM. Piperacillin-tazobactam is the most sensitive drug for middle ear infection. The most resistant drug is ciprofloxacin. [ABSTRACT FROM AUTHOR]