EXPRESSION OF SS AND SE GENES IN P. japonicus HAIR ROOTSAND ITS GINSENOSIDE RE SYNTHESIS.

In order to study the effect ofSS(squalene synthase) and SE (squalene epoxidase) genes co-over-expression in hairy roots of Panax japonicus on ginsenoside Resynthesis, the callus of P· japonicus was used as receptor material. The double expression vector of SS and SE genes were constructed respectively and transformed the P· japonicus callus by Agrobacterium LBA4404. Finally, C58Cl was used to induce hairy roots from Callus of transgenic P· japonicus. The results of PCR showed that SS and SE genes could be integrated into hairy roots of P· japonicus individually or jointly. RP-HPLC indicated that Re content in transgenic P·japonicus hairy roots was 2.4 times and 2.3 times of that in non-transgenic ones (CK) when SS and SE geneswere transferred alone. The Re content in transgenic P· japonicus hairy roots was 5.3 times that of the non-transgenic ones when SS and SE genes co-over-expression. It can be seen that the SS and SE genes has a positive regulatory effect on Re synthesis in P· japonicus hairy roots and the co-over-expression of SS and SE genes canincrease Re content. [ABSTRACT FROM AUTHOR]