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MiRNA-339-5p suppresses the malignant development of gastric cancer via targeting ALKBH1.
- Source :
-
Experimental & Molecular Pathology . Aug2020, Vol. 115, pN.PAG-N.PAG. 1p. - Publication Year :
- 2020
-
Abstract
- To uncover the role of microRNA-339-5p (miRNA-339-5p) in the development of gastric cancer (GC) and its possible molecular mechanism. Differential expressions of miRNA-339-5p in GC and adjacent normal tissues were detected. The relationship between miRNA-339-5p level and clinical features in GC patients was analyzed. Proliferative and migratory changes in BGC-823 and SGC-7901 cells overexpressing miRNA-339-5p were examined. Finally, luciferase assay and rescue experiments were conducted to explore the regulatory mechanism of miRNA-339-5p in its downstream gene ALKBH1, and their interaction in the development of GC. MiRNA-339-5p was downregulated in GC tissues. Lowly expressed miRNA-339-5p was unfavorable to prognosis in GC because of high rates of lymphatic metastasis and distant metastasis. Overexpression of miRNA-339-5p markedly reduced proliferative and migratory abilities in GC cells. ALKBH1 was identified to be the downstream gene of miRNA-339-5p. In GC tissues, ALKBH1 was upregulated and negatively correlated to miRNA-339-5p level. Overexpression of ALKBH1 was able to reverse the inhibitory effects of overexpressed miRNA-339-5p on proliferative and migratory abilities in GC. Lowly expressed miRNA-339-5p is closely related to metastasis and poor prognosis in GC patients. MiRNA-339-5p suppresses the malignant development of GC by negatively regulating ALKBH1. [ABSTRACT FROM AUTHOR]
- Subjects :
- *STOMACH cancer
*LYMPHATIC metastasis
Subjects
Details
- Language :
- English
- ISSN :
- 00144800
- Volume :
- 115
- Database :
- Academic Search Index
- Journal :
- Experimental & Molecular Pathology
- Publication Type :
- Academic Journal
- Accession number :
- 144622633
- Full Text :
- https://doi.org/10.1016/j.yexmp.2020.104449