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Production of styrene oxide from styrene by a recombinant Escherichia coli with enhanced AcrAB-TolC efflux pump level in an aqueous-organic solvent two-phase system.
- Source :
-
Bioscience, Biotechnology & Biochemistry . Jul2020, Vol. 84 Issue 7, p1513-1520. 8p. - Publication Year :
- 2020
-
Abstract
- The AcrAB-TolC efflux pump is involved in the organic solvent tolerance of Escherichia coli. Most E. coli strains are highly sensitive to organic solvents such as n-hexane and cyclohexane. Here, a recombinant E. coli transformed with an expression plasmid containing acrAB and tolC became tolerant to n-hexane and cyclohexane. The levels of AcrA, AcrB, and TolC in the recombinant increased by 3- to 5-fold compared to those in the control strain without the plasmid for acrAB or tolC. To investigate the usability of the recombinant as a biocatalyst in an aqueous-organic solvent two-phase system, we further introduced xylMA xylene monooxygenase genes from Pseudomonas putida mt-2 into the recombinant and examined the production of styrene oxide from styrene. The resulting recombinant produced 1.8 mg and 1.0 mg styrene oxide mL−1 of medium in a medium overlaid with a 25% volume of n-hexane and cyclohexane containing 10% (wt vol−1) styrene, respectively. A recombinant Escherichia coli with enhanced AcrAB-TolC efflux pump level increased production of styrene oxide from styrene in an aqueous-organic solvent two-phase system. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09168451
- Volume :
- 84
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Bioscience, Biotechnology & Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 144526476
- Full Text :
- https://doi.org/10.1080/09168451.2020.1755219