Positively Charged Polystyrene-co-Poly (ar-vinylbenzyl) Trimethylammonium Nanoparticles as Bioprobe for Various Cell Imaging Based on Aggregation Induced Emission.

In this study, positively charged polystyrene-co-poly (ar-vinylbenzyl) trimethylammonium nanoparticles loading with Aggregation Induced Emission (AIE) fluorescent molecule sulfonated 9,10-distyrylanthracene derivatives (SDSA) by coulomb force denoted as SDSA/PS-co-PVBTAC have been prepared. The size, size distribution and morphology of nanoparticles have been tested by Dynamic light scattering (DLS) and Scanning electron microscope (SEM). The synthesized nanoparticles possess uniform size, good monodispersity and low biological toxicity. The AIE-type SDSA/PS-co-PVBTAC showed good fluorescence stability at pH from 3 to 10. It has been proved that the biological macromolecules including Bovine Serum Albumin (BSA), DNA and RNA can amplify the cell imaging signals. Moreover, the AIE-type nanoparticles have been shown to perform fluorescent imaging of various cells. It suggests that the prepared SDSA/PS-co-PVBTAC can be used as a new biological probe in the field of cell imaging and detection, as well as expanding the application of AIE fluorescent molecules in biosensing field. Through Coulomb force of nanoparticles with AIE fluorophore SDSA, hybrid polymeric nanoparticles SDSA/PS-co-PVBTAC for various cells imaging were prepared. It has been proven that the biological macromolecules can amplify the fluorescence signals, accompanied with good fluorescence stability at pH from 3 to 10. The electrostatic attraction between the positively charged nanoparticles and the negatively charged cell membrane is conducive to improve the cellular uptake of nanoparticle, as well as the effect of intracellular imaging. [ABSTRACT FROM AUTHOR]